Research Paper Volume 15, Issue 3 pp 810—829
Comprehensive analysis of BUBs gene family in lung adenocarcinoma with immunological analysis
- 1 Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
- 2 Department of Nephrology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
- 3 Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, Guangdong, China
Received: October 12, 2022 Accepted: February 5, 2023 Published: February 13, 2023
https://doi.org/10.18632/aging.204517How to Cite
Copyright: © 2023 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Lung adenocarcinoma (LUAD) is one of the most commonly malignant tumors, and major challenges remain in the treatment of LUAD. Budding uninhibited by benzimidazole 1/3 (BUB1/3) play significant roles in the process of spindle-assembly checkpoint (SAC) during mitosis. However, their roles in LUAD have not been established. Here, we performed an immunological analysis of BUB1/3 in LUAD using a comprehensive bioinformatics approach, quantitative real-time-PCR and Western blotting technique. Our results indicated that the expression levels of BUB1 and BUB3 in LUAD samples were higher than the expression levels in the control groups and were associated with some clinicopathologic parameters in patients with LUAD. BUB1/3 and their related genes were enriched in cell immune, and the immune infiltration analysis revealed that the BUB1/3 expression profile was significantly correlated with characteristics of immune cell infiltration. Survival analysis showed that the disease-free survival and overall survival of patients with LUAD decreased with an increase in the BUB1/3 expression levels. The mRNA and protein expression levels of BUB1 and BUB3 in each of the LUAD cell lines were upregulated to varying degrees. BUB1 and BUB3 are the potential immunological therapeutic intervention targets for patients with LUAD.
Abbreviations
APC/C: Anaphase promoting complex C; AUC: Area Under Curve; BP: Biological process; BUB: Budding uninhibited by benzimidazole; CC: Cellular component; CPTAC: Clinical Proteomic Tumor Analysis Consortium; DEGs: Differential expression genes; DFS: Disease Free Survival; FPR: False positive rate; GEPIA: Gene Expression Profiling Interactive Analysis; GO: Gene Ontology; GTEx: Genotype-Tissue Expression; HPA: Human Protein Atlas; KEGG: Kyoto Encyclopedia of Genes and Genomes; LUAD: Lung adenocarcinoma; MAD: Mitotic arrest deficient; MF: Molecular function; MPS: Monopolar spindle; OS: Overall Survival; PPI: Protein-protein interaction; ROC: Receiver operating characteristic; SAC: spindle-assembly checkpoint; TCGA: The Cancer Genome Atlas; TIMER: Tumor Immune Estimation Resource; TPR: True positive rate; UALCAN: The University of Alabama at Birmingham cancer data analysis Portal.